INFLUENCE OF A FODDER ADDITIVE ON CHANGE OF BIODIVERSITY OF THE RUMEN MICROFLORA OF COWS

  • Евгений Олегович Крупин Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”
  • Шамиль Касымович Шакиров Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center «Kazan Scientific Center of Russian Academy of Sciences»
  • Марсель Шарипзянович Тагиров Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”
  • Анастасия Михайловна Харченко Kazan (Volga region) Federal University
  • Татьяна Владимировна Григорьева Kazan (Volga region) Federal University
Keywords: cow, digestion, microbiota, sequencing, DNA, metagenome

Abstract

The results of a comparative analysis of the microbiota of the rumen of four groups of dairy cows of the Kholmogory breed of the Tatarstan type are presented. The animals of the experimental groups were fed an experimental feed supplement in various doses as part of the feeding ration. The experimental feed additive in its composition contains enzymes, probiotic microorganisms, L-carnitine and sapropel taken in a certain ratio. The composition of the experimental feed additive is developed, and its required amount is produced in Tatar Research Institute of Agriculture of FRC Kazan Scientific Center of RAS. Analysis of the rumen microflora was performed by sequencing the 16S rRNA gene on the IlluminaMiSec platform in Kazan (Volga region) Federal University. Studies have established that the use of cows in the rations of feeding an experimental feed supplement did not have a visible effect on the composition of the rumen microflora as a whole. However, the use of experimental feed additive affected the content of important functional groups of microorganisms of the genera Fibrobacter, Ruminococcus, Anaeroplasma and Ruminobacter. These microorganisms provide carbohydrate metabolism, its intensity. The difference in the Shannon index gives grounds to believe that there is a possible dependence of the productive effect of the test feed additive on the uniformity of the distribution of bacteria in the rumen of the animals themselves. This research was supported by FASO Russia project АААА-А18-118031390148-1.

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Author Biographies

Евгений Олегович Крупин, Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”

Candidate of Veterinary Sciences

Шамиль Касымович Шакиров, Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center «Kazan Scientific Center of Russian Academy of Sciences»

Doctor of Agricultural Sciences

Марсель Шарипзянович Тагиров, Tatar Research Institute of Agriculture – Subdivision of the Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”

Doctor of Agricultural Sciences

Анастасия Михайловна Харченко, Kazan (Volga region) Federal University

Magistrandt

Татьяна Владимировна Григорьева, Kazan (Volga region) Federal University

Candidate of Biological Sciences

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Published
2018-12-10
Section
Естественные науки