• Екатерина Валерьевна Корниенко Centre of Experimental Embryology and Reproductive Biotechnology
  • Анастасия Борисовна Романова Centre of Experimental Embryology and Reproductive Biotechnology
  • Галина Петровна Маленко Centre of Experimental Embryology and Reproductive Biotechnology
Keywords: transgene, bovine, embryo, oocyte


In the current work we present a review of scientific literature concerning methods of obtaining trangenic bovine embryo. Based on the current data a conclusion can be made that all listed methods of transgenesis have certain limits in application to bovine oocytes and embryos that significantly reduce their effectiveness. The question of optimal method development is still open.


Download data is not yet available.

Author Biographies

Екатерина Валерьевна Корниенко, Centre of Experimental Embryology and Reproductive Biotechnology


Анастасия Борисовна Романова, Centre of Experimental Embryology and Reproductive Biotechnology

Junior Researcher

Галина Петровна Маленко, Centre of Experimental Embryology and Reproductive Biotechnology

Doctor of Biological Sciences


1. Bachiller D., Schellander K., Peli J., Ruthe U. Liposome-Mediated DNA Uptake by Sperm // Cells. Mol. Reprod. Dev.,1991, V. 30, P.194–200.
2. Bang J.I., Jin J.I., Ghanem N., Choi B.H., Fakruzzaman M., Ha A.N., Lee K.L., Uhm S.J., Ko D.H., Koo B.C. , Lee J.G., KongI. K. Quality improvement of transgenic cloned bovine embryos using an aggregation method: Effects on cell number, cell ratio, embryo perimeter, mitochondrial distribution, and gene expression profile // Theriogenology, 2015, V. 84, P. 509–523.
3. Bevacqua R.J., Pereyra-Bonnet F., Olivera R., Hiriart M.I., Sipowicz P., Fernandez-Martín R., Radrizzani M., Salamone D.F. Production of IVF transgeneexpressing bovine embryos using a novel strategy based on cell cycle inhibitors // Theriogenology, 2012, V. 78, P. 57–68.
4. Bi Y., Liu X., Zhang L., Shao C., Ma Z., Hua Z., et al. Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by streptomyces phage phiC31 integrase // BMC Mol Biol., 2013, V.14, P. 20.
5. Bordingnon V., Keyston R., Lazaris A., Bilodeau AS., Pontes J.H.F., Arnold D., Fecteau G., Keefer C., Smith L.C. Transgene expression of green fluorescent protein and germ line transmission in cloned calves derived from in vitro-transfected somatic cells // Biol Reprod, 2003, V.68, P. 2013–2023.
6. Fleming A., Abdalla E.A., Maltecca C., Baes C.F. Invited review: Reproductive and genomic technologies to optimize breeding strategies for genetic progress in dairy cattle // Arch. Anim. Breed.,2018, V. 61, P. 43–57.
7. Keefer C. L. Artificial cloning of domestic animals // P. Natl. Acad.Sci. USA, 2015, V.112, P. 8874–8878.
8. Monzani P.S., Guemra S., Adona P.R., Ohashi O.M. Working with Lentivirus for Generation of Transgenic Bovine Embryos. Pharm Anal Acta 5, 2014, P. 169.
9. Monzani P.S., Adona P.R., Ohashi O.M., Meirelles F.V., Wheeler M.B. Transgenic bovine as bioreactors: Challenges and perspectives // BIOENGINEERED, 2016, V. 7, No. 3, P. 123–131.
10. Niemann H., Kind A., Schienieke A. Productionofbio-pharmaceuticals in transgenic animals. // Kayser O,Warzecha H (eds), Pharmaceutical Biotechnology– Drug discovery and Clinical Applications, 2nd edn.Wiley-Blackwell, Weinheim, Germany, 2012, 71–111 pp.
11. Sekhavati M.H., Hosseini S.M., Tahmoorespur M., Ghaedi K., Jafarpour F., Hajian M., Dormiani K., Nasr-Esfahani M.H. PhiC31-based site-specific transgenesis system for production of transgenic bovine embryos by somatic cell nuclear transfer and intracytoplasmic sperm injection // Cell J., 2018, V. 20, No.1, P. 98–107.
12. Shemesh M., Gurevich M., Harel-Markowitz E., Benvenisti L., Shore L.S., Stram Y. Gene Integration Into Bovine Sperm Genome and Its Expression in Transgenic Offspring // Mol. Reprod. Dev., 2000, V. 56, P. 306–308.
13. Smith K. R. Sperm-Mediated Gene Transfer: Concepts and Controversies. – Bentham Science Publishers. 2012.
14. Spadafora C. Sperm-mediated ‘reverse’ gene transfer: a role of reverse transcriptase in the generation of new genetic information // Human Reproduction, 2008,P. 735–740.
15. Suzuki R., Takizawa T., Negishi Y., Utoguchi N., Maruyama K. Effective gene delivery with novel liposomal bubbles and ultrasonic destruction technology // International Journal of Pharmaceutics, 2008, V. 354, P. 49–55.
16. Vajta G., Peura T.T., Holm P., Páldi A., Greve T., Trounson A.O., Callesen H. New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system // Mol. Reprod. Dev., 2000, V. 55, P. 256–264.
17. Vichera G., Moro L., Salamone D. Efficient Transgene Expression in IVF and Parthenogenetic Bovine Embryos by Intracytoplasmic Injection of DNA–Liposome Complexes // Reprod. Dom. Anim., 2011, V. 46, No. 2, P. 214–220.
18. Vichera G., Moro L.N., Buemo C., Salamone D. DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA–liposome complexes in IVF bovine zygotes // Zygote , 2012, V. 22, No.02, P. 195–203.
19. Vichera G., Olivera R., Salamone D. Oocyte genome cloning used in biparental bovine embryo reconstruction // Zygote, 2013, V. 21, No.01, P. 21–29.
20. Wall R.J. Pronuclear microinjection. // Cloning Stem Cells, 2001, V. 3, P. 209–220.
21. Wang Y.S., Tang S., An Z.X., Li W.Z., Liu J., Quan F.S., et al. Effect of mSOF and G1.1/G2.2 media on the developmental competence of SCNTderived bovine embryos // Reprod Domest Anim, 2011, V. 46, P. 404–409.
Естественные науки