EVALUATION OF C337G POLYMORPHISM OF FSHR GENE IN THE POPULATION OF BLACK0AND-WHITE HOLSTEINIZED CATTLE

  • Анна Владимировна Бабий Center of Experimental Embryology and Reproductive Biotechnologies
  • Анна Леонидовна Архипова Center of Experimental Embryology and Reproductive Biotechnologies
  • Сергей Алексеевич Бурсаков Center of Experimental Embryology and Reproductive Biotechnologies
  • Артем Владимирович Бригида Center of Experimental Embryology and Reproductive Biotechnologies
  • Евгений Александрович Климов Center of Experimental Embryology and Reproductive Biotechnologies
  • Светлана Николаевна Ковальчук Center of Experimental Embryology and Reproductive Biotechnologies
Keywords: allelic polymorphism, FSHR, cattle, PCR-RFLP

Abstract

One of the crucial step of the technology of accelerated reproduction of cattle is selection of donor cows, which are the most sensitive to the procedure of hormonal stimulation of ovulation. To date, one of the promising genetic markers of the reproductive status of cattle is the follicle-stimulating hormone receptor (FSHR) gene. Special attention is drawn to SNP C337G (rs43745234), associated with the number of fertilized oocytes and viable embryos. The aim of this work was to evaluate the polymorphism C337G of the FSHR gene in a population of Black-and-White holsteinized cattle. The article presents data on genotyping of 190 Black-and-White holsteinized cows by the allelic variants C337G of FSHR gene (rs43745234) by PCR-RFLP method. Analysis of the results showed that in the studied cattle population C and G allele frequencies were 0,7 and 0,3. The frequencies of genotypes C/C and C/G  were 474 and 0,453, respectively. The frequencies of G/G genotype associated with a large number of viable embryos (according to literature data) was only 0,074.

 

 

Downloads

Download data is not yet available.

Author Biographies

Анна Владимировна Бабий, Center of Experimental Embryology and Reproductive Biotechnologies

Candidate of Biological Sciences

Анна Леонидовна Архипова, Center of Experimental Embryology and Reproductive Biotechnologies

junior researcher

Сергей Алексеевич Бурсаков, Center of Experimental Embryology and Reproductive Biotechnologies

Candidate of Biological Sciences

Артем Владимирович Бригида, Center of Experimental Embryology and Reproductive Biotechnologies

Researcher

Евгений Александрович Климов, Center of Experimental Embryology and Reproductive Biotechnologies

Doctor of Biological Sciences

Светлана Николаевна Ковальчук, Center of Experimental Embryology and Reproductive Biotechnologies

Candidate of Biological Sciences

References

1. Бурсаков С.А. и др. Генетические маркеры суперовуляторного ответа у крупного рогатого скота // Проблемы биологии продуктивных животных. – 2017. – № 4. – С. 5–23.
2. Berg H. Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments (PCR-RFLP) and Gel Electrophoresis – Valuable Tool for Genotyping and Genetic Fingerprinting // Gel Electrophoresis – Principles and Basics. – InTech. – 2012.
3. Bó G.A., Mapletoft R.J. Historical perspectives and recent research on superovulation in cattle // Theriogenology. – 2014. Vol. 81 – P. 38–48.
4. Choi Y., Chan A.P. PROVEAN web server: A tool to predict the functional effect of amino acid substitutions and indels // Bioinformatics. – 2015. – Vol. 31. – P. 2745–2747.
5. Cory A.T., Price C.A., Lefebvre R., Palin M.F. Identification of single nucleotide polymorphisms in the bovine follicle-stimulating hormone receptor and effects of genotypes on superovulatory response traits // Animal Genetics. – 2013. – Vol. 44. – P. 197–201.
6. Fauser B., Diedrich K., Devroey P. Predictors of ovarian response: Progress towards individualized treatment in ovulation induction and ovarian stimulation // Human Reproduction Update. – 2008. – Vol. 14. – P. 1–14.
7. GeneRunner http://www.generunner.net/
8. Gromoll J., Simoni M. Genetic complexity of FSH receptor function // Trends in Endocrinology and Metabolism. – 2005. – Vol. 16. – P. 368–373.
9. Hamny H. et al. Polymorphism of follicle stimulating hormone receptor influences the 3D structure and its binding pattern to FSH in Bos taurus // Indian Journal of Animal Research. – 2017. – Vol. 51. – P. 630–634.
10. Hasler J.F. Forty years of embryo transfer in cattle: A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces // Theriogenology. – 2014. Vol. 81. – P. 152–169.
11. Houde A. et al. Structure of the bovine follicle-stimulating hormone receptor complementary DNA and expression in bovine tissues // Molecular Reproduction and Development. – 1994. – Vol. 39. – P. 127–35.
12. Joshi A.D., Pajor A.M. Role of conserved prolines in the structure and function of the Na+/dicarboxylate cotransporter 1, NaDC1 // Biochemistry. – 2006. – Vol. 45. – P. 4231–4239.
13. Kroeze W.K., Sheffler D.J., Roth B.L. G-protein-coupled receptors at a glance // Journal of Cell Science. – 2003. – Vol. 116. – P. 4867–4869.
14. Mapletoft R.J., Hasler J.F. Assisted reproductive technologies in cattle: a review // Revue scientifique et technique. – 2005. – Vol. 24. – P. 393–403.
15. Milazzotto M.P. et al. New molecular variants of hypothalamus-pituitary-gonad axis genes and their association with early puberty phenotype in Bos taurus indicus (Nellore) // Livestock Science. – 2008. – Vol. 114. – P. 274–279.
16. Mossa F. et al. Association between numbers of ovarian follicles in the first follicle wave and superovulatory response in ewes // Animal Reproduction Science. – 2007. – Vol. 100. – P. 391–396.
17. Nicholson H., Tronrud D.E., Becktel W.J., Matthews B.W. Analysis of the effectiveness of proline substitutions and glycine replacements in increasing the stability of phage T4 lysozyme // Biopolymers. – 1992. – Vol. 32. – P. 431–441.
18. Rahal P. et al. Polymorphisms in the bovine follicle-stimulating hormone receptor gene // Animal Genetics – 2000. – Vol. 31. – P. 280–281.
19. Tang K.Q., Yang W.C., Li S.J., Yang L.G. Polymorphisms of the bovine growth differentiation factor 9 gene associated with superovulation performance in Chinese Holstein cows // Genetics and Molecular Research. – 2013. – Vol. 12. – P. 390–399.
20. Yang W.C., Li S.J., Chen L., Yang L.G. FSHR genotype affects estrogen levels but not pregnancy rates in Luxi cattle subjected to embryo transfer // Genetics and Molecular Research. – 2014. – Vol. 13. – P. 1563–1569.
Published
2018-12-10
Section
Естественные науки