Application of RT-PCR method for the determination of fungal and bacterial dna in the preservation of green mass of alfalfa with Lacticaseibacillus paracasei and Lactiplantibacillus plantarum
DOI:
https://doi.org/10.28983/asj.y2021i10pp73-76Keywords:
RT-PCR, alfalfa, silage, microorganisms, lactobacilli, fungi, DNAAbstract
New Lactobacillus strains, Lacticaseibacillus paracasei L1a and Lactiplantibacillus plantarum S1a R were isolated from bovine feces were used as bioconservants for Medicago sativa (alfalfa) silage after 16S rRNA identification. After 60 days the fungal content in the silage was defined by the method of qPCR using primers based on the ?-actin gene of fungi. L1a and S1a R were able to reduce fungal DNA after ensilaging by 93.7% and 95.3% respectively in comparison to control. Total lactobacillus content in the silage was also determined and results showed a high DNA content of lactic acid bacteria in L1a samples (0.00468 ng) followed by S1a R samples (0.00245 ng) when compared to control samples (0.00176 ng). The ability of these strains to reduce fungi population and the same time increase Lactic acid bacteria is an indicator for the post-conservation of silage and can be used for bio preparations.
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