Marker selection for genes Rvi6, Md-ACS1, Md-ACO1 promising for breeding of apple gene pool samples

Abstract

The article presents data of molecular-genetic analysis of collection varieties of apple (Malus ? domestica Borkh.) and new hybrid material of various genetic and ecological-geographical origin on the gene Rvi6 resistance to scab (Venturia equalinais (Cooke) G. Winter) and target alleles of the Md-ACS1, Md-ACO1 genes, which cause increased fruit density. During the research work, the СТАВ method was used with a modification earlier in the NCFSCHVW for more complete purification of DNA samples during extraction. SCAR-markers were used: Rvi6 – VfC; Md-ACS1, Md-ACO1. The experiment was carried out with the participation of 26 samples of apple trees, among them: 11 new domestic varieties, 2 varieties of foreign breeding and 13 hybrid forms of breeding NCFSCHVW. With the help of marker selection, carriers of the gene Rvi6 resistant to scab have been selected: Nadezhnoye, Mikhsan, Gayto Gazdanov and 5 hybrids, most of which are created with the participation of the variety Modi. An allele combination of Md-ACO1-1/1 has been found in Red Delicious King Roat. Combinations of Rvi6, Md-ACS1-2/2 genes have been identified: Nadezhnoye and hybrid form 17/1-7-26 from the Champion ? Modi family; these genotypes of domestic breeding are promising for inclusion in the further breeding process.