Effects of highly dispersed silica nanoparticles on the cryoresistance of devitrified Bos taurus cumulus-oocyte complexes
DOI:
https://doi.org/10.28983/asj.y2019i3pp29-34Keywords:
oocytes, in vitro, highly dispersed silica nanoparticles, сumulus, vitrification, BosTaurusAbstract
The creation of the donor oocytes cryobank in agricultural animals will make it possible to significantly improve the introduction of high-tech cellular reproductive and DNA technologies (in vitro production of embryos, cloning, transgenesis, genome editing) into the animal husbandry practice. Despite the considerable efforts of cryobiologists and embryotechnologists, the problem of Bos Taurus oocyte cryoresistance has not been adequately solved. The search for effective cryoprotective agents (CPA) that preserve the integrity of cellular structures, their functional activity and the creation of a system for the cultivation of devitrified cells are the most important tasks of modern cryotechnologies. Highly dispersed silica nanoparticles (HDSns) are promising components for optimizing CPA systems and media for cultivating somatic and germ cells of ovarian follicles, as well as pre-implantation embryos of animals. In the present study the nature of the effect of HDSns on the somatic (cumulus), germ cells (oocytes) of ovarian cow follicles and the fertility of the ova was identified. The positive effects of HDSns on the safety and functional state of cumulus cells after devitrification and cultivation of cumulus - oocyte complexes were shown. The level of devitrified oocytes that have cultured with 0.001% of HDSns and have reached the metaphase II, significantly exceeded the level of matured devitrified oocytes that have not treated with HDSns (60% vs. 41 %, P<0.001 (?2-test)).The yield of embryos at the blastocyst stage significantly increased when devitrified oocytes were cultured with HDSns (11% (13/121) vs. 5% (5/105), P <0.05). In addition to the observed positive effects of the HDSns on the cumulus morphology and maturation of devitrified Bos Taurus oocytes, the data obtained contribute to a better understanding of the mechanisms of functioning of somatic and germ cells from ovarian follicles under ultralow temperatures.
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