Assessment of destructive processes of chromatin in granulose of porcine ovarian fol-licules containing growing or fully grown oocytes
DOI:
https://doi.org/10.28983/asj.v0i9.578Keywords:
porcine granulosa, oocyte, BCB-test, apoptosisAbstract
Significant differences in character of atretic processes in porcine granulosa were established as a result of comparative monitoring of the viability and degeneration of chromatin (apoptosis, pycnosis) in granulosa cells from follicles containing growing or fully grown oocytes using cytology analysis, cytofluorometry (Annexin-test) and TUNEL-test. BCB-test was used for the evaluation of the functional state of the oocytes (EgerszegiI. etal., 2010). Completion of the oocyte growth phase was accompanied by a significant increase in the proportion of granulosa cells from the follicles with the fully grown oocyte in the status of apoptosis (11% vs 7%, P <0.05), pyknosis (9% vs 5%, P <0.05), necrosis (5% vs 3%, P <0.05) and a decreasing in the level of viable cells (85% vs 88, P <0.05) compared to the above markers in granulosa cells from follicles with growing oocytes. The revealed features of destructive processes in granulosa cells from follicles containing growing or fully grown oocytes supplement the available information on the features of the interaction of the germ and somatic cells of the ovarian follicle in the formation of an egg that is capable of fertilization and further development of the embryo.
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