INFLUENCE OF A FODDER ADDITIVE ON CHANGE OF BIODIVERSITY OF THE RUMEN MICROFLORA OF COWS
DOI:
https://doi.org/10.28983/asj.v0i12.537Keywords:
cow, digestion, microbiota, sequencing, DNA, metagenomeAbstract
The results of a comparative analysis of the microbiota of the rumen of four groups of dairy cows of the Kholmogory breed of the Tatarstan type are presented. The animals of the experimental groups were fed an experimental feed supplement in various doses as part of the feeding ration. The experimental feed additive in its composition contains enzymes, probiotic microorganisms, L-carnitine and sapropel taken in a certain ratio. The composition of the experimental feed additive is developed, and its required amount is produced in Tatar Research Institute of Agriculture of FRC Kazan Scientific Center of RAS. Analysis of the rumen microflora was performed by sequencing the 16S rRNA gene on the IlluminaMiSec platform in Kazan (Volga region) Federal University. Studies have established that the use of cows in the rations of feeding an experimental feed supplement did not have a visible effect on the composition of the rumen microflora as a whole. However, the use of experimental feed additive affected the content of important functional groups of microorganisms of the genera Fibrobacter, Ruminococcus, Anaeroplasma and Ruminobacter. These microorganisms provide carbohydrate metabolism, its intensity. The difference in the Shannon index gives grounds to believe that there is a possible dependence of the productive effect of the test feed additive on the uniformity of the distribution of bacteria in the rumen of the animals themselves. This research was supported by FASO Russia project АААА-А18-118031390148-1.
Downloads
References
2. Влияние экспериментальной кормовой добавки на активность ферментов сыворотки крови и показатели рубцовой жидкости коров / Е.О Крупин, Ш.К. Шакиров, Т.В. Жарехина, М.Ш. Тагиров // Вестник Казанского ГАУ. – 2018. – №2 (49). – С. 39 – 42.
3. Григорьев, В.С. Ростовые и биологические особенности телят при разных методах кормления / В.С. Григорьев, Л.Н. Бакаева // Известия Самарской ГСХА. – 2012. – №1. – С. 103 – 107.
4. Киселев, С. Полноценное кормление коров / С. Киселев, М. Петухов // Животноводство России. – 2005. – № 6. – С. 47 – 48.
5. Методы ветеринарной клинической лабораторной диагноcтики: Справочник / Кондрахин И.П., Архипов А.В., Левченко В.И., Таланов Г.А., Фролова Л.А., Новиков В.Э. – М.: Колос. – 2004. – 520 c.
6. Пристеночная микрофлора кишечника / И.Д. Лоранская, М.Н. Болдырева, О.А. Лаврентьева, Э.В. Мулухова. – Москва: Прима Принт, 2015. – 100с.
7. Evaluation of 16S rRNA Gene Primer Pairs for Monitoring Microbial Community Structures Showed High Reproducibility within and Low Comparability between Datasets Generated with Multiple Archaeal and Bacterial Primer Pairs / M. A. Fischer, S. G?llert, S. C. Neulinger, W. R. Streit, R. A. Schmitz // Frontiers in Microbiology. – 2016. – V.7. – 1297 p.
8. Hungate, R.E. A roll tube method for cultivation of strict anaerobes. In: Norris J. R., Ribbons D. W. (eds). Methods in microbiology, vol. 3B. Academic Press, London and New York, p. 117–132, 1969.
9. Uyeno, Y.T. Effect of Probiotics/Prebiotics on Cattle Health and Productivity / Y. Uyeno, S. Shigemori, T.Shimosato // Microbes and Environments. – 2015. – V.30. – N2. – P. 126 – 132.
10. Web based phylogenetic assignment tool for analysis of terminal restriction fragment length polymorphism profiles of microbial communities / A.D. Kent, D.J. Smith, B.J. Benson, E.W. Triplett // Appl. Environ. Microbiol. – 2003. – Vol. 69. – P. 6768 – 6776